home *** CD-ROM | disk | FTP | other *** search
/ Celestin Apprentice 5 / Apprentice-Release5.iso / Source Code / Libraries / DCLAP 6d / dclap6d / SeqPups / seqs / testre.map < prev    next >
Text File  |  1996-07-05  |  30KB  |  524 lines

  1.  (Linear) MAP of: testre.gcg  check: 2116  from: 1  to: 1501
  2.  
  3.  
  4.  With 209 enzymes: * 
  5.  
  6.  MaxCuts: 4 
  7.                            July 1, 1995  12:42  ..
  8.  
  9.                          S  E     B
  10.                          a  c H  Ep
  11.                     A    u Do i  cu                FH    M        N
  12.                     l    3 pR n  o1                oh    s        s
  13.                     w    A nI 4  N0                ka    e        p
  14.                     I    I II I  II                II    I        I
  15.                                                     /              
  16.          ATATAACATGAGAGTTTGATCCTGGCTCAGGATGAACGCTGCGCGTGCTTAACACATGCA
  17.        1 ---------+---------+---------+---------+---------+---------+ 60
  18.          TATATTGTACTCTCAAACTAGGACCGAGTCCTACTTGCGACGCGCACGAATTGTGTACGT
  19.  
  20. a        I  *                                                         -
  21. b         Y  N  M  R  V  *              M  N  A  A  R  A  *     M  Q  -
  22. c          I  T  *                                                    -
  23.        1 ---------+---------+---------+---------+---------+---------+ 60
  24. d                                                                     -
  25. e                                                                     -
  26. f          Y  L  M                                         *  C  M  C -
  27.  
  28.                                S
  29.                                a
  30.                           A    u D                                 E
  31.                           l    3 p                                 c
  32.                           w    A n                                 i
  33.                           I    I I                                 I
  34.          AGTCGAACGAAGCATTTACTTACGATCCCTTCGGGGTGACGAGTTTATGACTGAGTGGCG
  35.       61 ---------+---------+---------+---------+---------+---------+ 120
  36.          TCAGCTTGCTTCGTAAATGAATGCTAGGGAAGCCCCACTGCTCAAATACTGACTCACCGC
  37.  
  38. a                                                                     -
  39. b         V  E  R  S  I  Y  L  R  S  L  R  G  D  E  F  M  T  E  W  R  -
  40. c                                                                     -
  41.       61 ---------+---------+---------+---------+---------+---------+ 120
  42. d                                                                     -
  43. e                                                                     -
  44. f          T  S  R  L  M                                              -
  45.  
  46.                      A      B            B
  47.                      f      s            sE                   B
  48.                      lM     t         M  pc                   s     C
  49.                      Il     E         m  2o                   m     j
  50.                      Iu     I         e  4N                   F     e
  51.                      II     I         I  II                   I     I
  52.                       /                   /                          
  53.          GACGGGTGAGTAACGCGTGGGTAACCTACCTTATACAGGGGGACAACAGTTGGAAACGAC
  54.      121 ---------+---------+---------+---------+---------+---------+ 180
  55.          CTGCCCACTCATTGCGCACCCATTGGATGGAATATGTCCCCCTGTTGTCAACCTTTGCTG
  56.  
  57. a                                                                     -
  58. b         T  G  E  *                                                  -
  59. c                                                                     -
  60.      121 ---------+---------+---------+---------+---------+---------+ 180
  61. d                                                                     -
  62. e                                                                     -
  63. f                                                                     -
  64.  
  65.              B                               A
  66.          C   s                               f
  67.          j   p             H                 l
  68.          e   2             h                 I
  69.          P   4             a                 I
  70.          I   I             I                 I
  71.          /                                    
  72.          TGCTAATACCGCATAAGCGCACGATGTTGCATGACAACGTGTGAAAAGATTTATCGGTAT
  73.      181 ---------+---------+---------+---------+---------+---------+ 240
  74.          ACGATTATGGCGTATTCGCGTGCTACAACGTACTGTTGCACACTTTTCTAAATAGCCATA
  75.  
  76. a                                      M  T  T  C  E  K  I  Y  R  Y   -
  77. b                                                                     -
  78. c                               M  L  H  D  N  V  *                   -
  79.      181 ---------+---------+---------+---------+---------+---------+ 240
  80. d                                                                     -
  81. e            *  Y  R  M  L  A  R  H  Q  M                             -
  82. f                                                                     -
  83.  
  84.                S                                                S
  85.               Aa   P                                           Ba
  86.          H   Bvu   s     F                             S       cu D
  87.          g   ca9   h     a                             t       e3 p
  88.          a   cI6   A     u                             y       fA n
  89.          I   III   I     I                             I       II I
  90.                /                                                   
  91.          AAGATGGACCCGCGTCTGATTAGCTAGTTGGTGAGGTAACGGCTCACCAAGGCGACGATC
  92.      241 ---------+---------+---------+---------+---------+---------+ 300
  93.          TTCTACCTGGGCGCAGACTAATCGATCAACCACTCCATTGCCGAGTGGTTCCGCTGCTAG
  94.  
  95. a        K  M  D  P  R  L  I  S  *                                    -
  96. b                                                                     -
  97. c                                                                     -
  98.      241 ---------+---------+---------+---------+---------+---------+ 300
  99. d                                                                     -
  100. e                                 *  N  T  L  Y  R  S  V  L  A  V  I  -
  101. f                                                                     -
  102.  
  103.                       H   B       H                    S H
  104.                       g   s   B  Ga       B            aBa
  105.                       i   t   s Ede       s            use
  106.                       E   E   r aiI       m            9mI
  107.                       I   I   F eII       A            6FI
  108.                       I   I   I III       I            III
  109.                                  /                       /
  110.          AGTAGCCGACCTGAGAGGGTGACCGGCCACATTGGGACTGAGACACGGCCCAAACTCCTA
  111.      301 ---------+---------+---------+---------+---------+---------+ 360
  112.          TCATCGGCTGGACTCTCCCACTGGCCGGTGTAACCCTGACTCTGTGCCGGGTTTGAGGAT
  113.  
  114. a                                                                     -
  115. b                                                                     -
  116. c                                                                     -
  117.      301 ---------+---------+---------+---------+---------+---------+ 360
  118. d                                                                     -
  119. e         L  L  R  G  S  L  T  V  P  W  M                             -
  120. f                                                                     -
  121.  
  122.          BE              T                S                  B
  123.          cc              s   S            f H                s  T
  124.          eo              p   s            a h                a  a
  125.          fN              R   p            N a                H  u
  126.          II              I   I            I I                I  I
  127.           /                                                      
  128.          CGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATGCAGCGACGCCGCG
  129.      361 ---------+---------+---------+---------+---------+---------+ 420
  130.          GCCCTCCGTCGTCACCCCTTATAACGTGTTACCCGCGTTCGGACTACGTCGCTGCGGCGC
  131.  
  132. a                                                                     -
  133. b                                                                     -
  134. c                                     M  G  A  S  L  M  Q  R  R  R  V -
  135.      361 ---------+---------+---------+---------+---------+---------+ 420
  136. d                                                                     -
  137. e                                                                     -
  138. f                                                                     -
  139.  
  140.                           M                                     M
  141.          H           X    b                                    BbR K
  142.          g           m    o                                    aos p
  143.          a           n    I                                    nIa n
  144.          I           I    I                                    III I
  145.                                                                 /   
  146.          TGAGCGAAGAAGTATTTCGGTATGTAAAGCTCTATCAGCAGGGAAGATAATGACGGTACC
  147.      421 ---------+---------+---------+---------+---------+---------+ 480
  148.          ACTCGCTTCTTCATAAAGCCATACATTTCGAGATAGTCGTCCCTTCTATTACTGCCATGG
  149.  
  150. a                             M  *                                    -
  151. b                                                         M  T  V  P  -
  152. c          S  E  E  V  F  R  Y  V  K  L  Y  Q  Q  G  R  *             -
  153.      421 ---------+---------+---------+---------+---------+---------+ 480
  154. d                                                                     -
  155. e                                                                     -
  156. f                                                                     -
  157.  
  158.                                               M
  159.                      B           B            sS       BS
  160.                      s           s          DTpa       sn
  161.                      r           a          saAc       aa
  162.                      F           A          au1I       AB
  163.                      I           I          IIII       II
  164.                                              /          /
  165.          TGACTAAGAAGCACCGGCTAAATACGTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGC
  166.      481 ---------+---------+---------+---------+---------+---------+ 540
  167.          ACTGATTCTTCGTGGCCGATTTATGCACGGTCGTCGGCGCCATTATGCATACCACGTTCG
  168.  
  169. a                                                                     -
  170. b         D  *                                            M  V  Q  A  -
  171. c                                                                     -
  172.      481 ---------+---------+---------+---------+---------+---------+ 540
  173. d                                                                     -
  174. e                                                                     -
  175. f                                                                     -
  176.  
  177.              BBT
  178.              ssa        B  B
  179.              apq        f  s
  180.              WEI        i  r
  181.              III        I  I
  182.               //            
  183.          GTTATCCGGATTTACTGGGTGTAAAGGGAGCGTAGGCGGTTTTACAAGTCTGATGTGAAA
  184.      541 ---------+---------+---------+---------+---------+---------+ 600
  185.          CAATAGGCCTAAATGACCCACATTTCCCTCGCATCCGCCAAAATGTTCAGACTACACTTT
  186.  
  187. a                                                                     -
  188. b         L  S  G  F  T  G  C  K  G  S  V  G  G  F  T  S  L  M  *     -
  189. c                                                                     -
  190.      541 ---------+---------+---------+---------+---------+---------+ 600
  191. d                                                                     -
  192. e                                                                     -
  193. f                                                                     -
  194.  
  195.                        C
  196.            D          Dj              M   S
  197.            s          se              m   f
  198.            a          aP              e   c
  199.            I          II              I   I
  200.          GCCCGTGGCTCAACCACGGTATTGCATTGGAAACTGTAGAACTAGAGTGTCGGAGGGGTA
  201.      601 ---------+---------+---------+---------+---------+---------+ 660
  202.          CGGGCACCGAGTTGGTGCCATAACGTAACCTTTGACATCTTGATCTCACAGCCTCCCCAT
  203.  
  204. a                                                                     -
  205. b                                                                     -
  206. c                                                                     -
  207.      601 ---------+---------+---------+---------+---------+---------+ 660
  208. d                                                                     -
  209. e                                                                     -
  210. f                   *  G  R  Y  Q  M                                  -
  211.  
  212.                T
  213.                s
  214.               Ep                                                 T
  215.              Ac5                                          B      s
  216.              po0                                          s      p
  217.              oR9                                          r      R
  218.              III                                          I      I
  219.               //                                                  
  220.          AGTGGAATTCCTAGTGTAGCGGTGAAATGCGTAGATATTAGGAGGAACACCAGTGGCGAA
  221.      661 ---------+---------+---------+---------+---------+---------+ 720
  222.          TCACCTTAAGGATCACATCGCCACTTTACGCATCTATAATCCTCCTTGTGGTCACCGCTT
  223.  
  224. a                                                                     -
  225. b                                                                     -
  226. c                                  M  R  R  Y  *                      -
  227.      661 ---------+---------+---------+---------+---------+---------+ 720
  228. d                                                                     -
  229. e                                                                     -
  230. f                                                                     -
  231.  
  232.                                    B
  233.            C        B              p
  234.            jT       s B            u    H
  235.            ea       p s            1    g
  236.            Pu       G r            0    a
  237.            II       I I            I    I
  238.             /                            
  239.          GGCGGCTTACTGGACGATTACTGACGCTGAGGCTCGAAAGCGTGGGGAGCAAACAGGATT
  240.      721 ---------+---------+---------+---------+---------+---------+ 780
  241.          CCGCCGAATGACCTGCTAATGACTGCGACTCCGAGCTTTCGCACCCCTCGTTTGTCCTAA
  242.  
  243. a                                                                     -
  244. b                                                                     -
  245. c                                                                     -
  246.      721 ---------+---------+---------+---------+---------+---------+ 780
  247. d                                                                     -
  248. e                           *  Q  R  Q  P  E  F  A  H  P  A  F  L  I  -
  249. f                                                                     -
  250.  
  251.                  T
  252.                  t
  253.                 Eh                                  B
  254.               B c1                            C     s             H
  255.               cBo1                           Cj    Bp             i
  256.               eaR1                           je    s2             n
  257.               feII                           eP    b4             f
  258.               IIII                           II    II             I
  259.                 //                            /     /              
  260.          AGATACCCTGGTAGTCCACGCCGTAAACGATGAATACTAGGTGTTGGCTTCCATAGGGAG
  261.      781 ---------+---------+---------+---------+---------+---------+ 840
  262.          TCTATGGGACCATCAGGTGCGGCATTTGCTACTTATGATCCACAACCGAAGGTATCCCTC
  263.  
  264. a                                                                     -
  265. b                                                                     -
  266. c                                     M  N  T  R  C  W  L  P  *       -
  267.      781 ---------+---------+---------+---------+---------+---------+ 840
  268. d                                                                     -
  269. e         L  Y  G  P  L  G  R  R  L  R  H  I  S  P  T  P  K  W  L  S  -
  270. f                                              *  T  N  A  E  M       -
  271.  
  272.                        B                E
  273.                        s                c
  274.            B  PT       p                o         R
  275.            a  la       2                R         s
  276.            n  eu       4                I         a
  277.            I  II       I                I         I
  278.          TCGGTGCCGCAGCTAACGCAATAAGTATTCCACCTGGGGAGTACGTTCGCAAGAATGAAA
  279.      841 ---------+---------+---------+---------+---------+---------+ 900
  280.          AGCCACGGCGTCGATTGCGTTATTCATAAGGTGGACCCCTCATGCAAGCGTTCTTACTTT
  281.  
  282. a                                                              M  K   -
  283. b                                                                     -
  284. c                                                                     -
  285.      841 ---------+---------+---------+---------+---------+---------+ 900
  286. d                                                                     -
  287. e         D  T  G  C  S  V  C  Y  T  N  W  R  P  L  V  N  A  L  I  F  -
  288. f                      *  R  L  L  Y  E  V  Q  P  T  R  E  C  S  H  F -
  289.  
  290.                            E
  291.                 T          c                            T
  292.                 s          oP S                         s
  293.                 p         AOsSa        B                p
  294.                 5         v1pau      F s          NC   M5   N
  295.                 0         a05n9      a m          sj   s0   s
  296.                 9         I9ID6      u F          pe   e9   p
  297.                 I         IIIII      I I          II   II   V
  298.                            ////                              
  299.          CTCAAAGGAATTGACGGGGACCCGCACAAGCGGTGGAGCATGTGGTTTAATTCGAAGCAA
  300.      901 ---------+---------+---------+---------+---------+---------+ 960
  301.          GAGTTTCCTTAACTGCCCCTGGGCGTGTTCGCCACCTCGTACACCAAATTAAGCTTCGTT
  302.  
  303. a        L  K  G  I  D  G  D  P  H  K  R  W  S  M  W  F  N  S  K  Q   -
  304. b                                                                     -
  305. c                                                                     -
  306.      901 ---------+---------+---------+---------+---------+---------+ 960
  307. d                                                        *  N  S  A   -
  308. e         S  L  P  I  S  P  S  G  C  L  R  H  L  M                    -
  309. f          E  F  S  N  V  P  V  R  V  L  P  P  A  H  P  K  I  R  L  L -
  310.  
  311.                         M                                 M
  312.                     F   b                                 b
  313.                     o   o                                 o
  314.                     k   I                                 I
  315.                     I   I                                 I
  316.          CGCGAAGAACCTTACCAAATCTTGACATCCTCTTGACAAACTATGTAATGTAGTCTTTCT
  317.      961 ---------+---------+---------+---------+---------+---------+ 1020
  318.          GCGCTTCTTGGAATGGTTTAGAACTGTAGGAGAACTGTTTGATACATTACATCAGAAAGA
  319.  
  320. a        R  E  E  P  Y  Q  I  L  T  S  S  *        M  *               -
  321. b                                                                     -
  322. c                                                       M  *          -
  323.      961 ---------+---------+---------+---------+---------+---------+ 1020
  324. d        V  R  L  V  K  G  F  R  S  M                                 -
  325. e                                                                     -
  326. f          A  F  F  R  V  L  D  Q  C  G  R  S  L  S  H  L  T  T  K  R -
  327.  
  328.                                                          A
  329.                                                 B        cB
  330.                      B                          s      B es
  331.                      c                          s      c Im
  332.                      g                          S      g IF
  333.                      I                          I      I II
  334.          TCGGAACAAGAGTGACAGGTGGTGCATGGTTGTCGTCAGCTCGTGTCGTGAGATGTTGGG
  335.     1021 ---------+---------+---------+---------+---------+---------+ 1080
  336.          AGCCTTGTTCTCACTGTCCACCACGTACCAACAGCAGTCGAGCACAGCACTCTACAACCC
  337.  
  338. a                                                                     -
  339. b                                 M  V  V  V  S  S  C  R  E  M  L  G  -
  340. c                                                                     -
  341.     1021 ---------+---------+---------+---------+---------+---------+ 1080
  342. d                                                                     -
  343. e                                                                     -
  344. f          R  F  L  L  S  L  H  H  M                                  -
  345.  
  346.                                                                  B
  347.                                                                  s
  348.                                                                  p
  349.                                                                  1 B
  350.          M             F   H                                   B 2 s
  351.          s             a   h                                   m 8 m
  352.          e             u   a                                   g 6 A
  353.          I             I   I                                   I I I
  354.          TTAAGTCCCGCAACGAGCGCAACCCTTGTCCTTAGTAGCCAGCATTTTAGGTGGGCACTC
  355.     1081 ---------+---------+---------+---------+---------+---------+ 1140
  356.          AATTCAGGGCGTTGCTCGCGTTGGGAACAGGAATCATCGGTCGTAAAATCCACCCGTGAG
  357.  
  358. a                                                                     -
  359. b         *                                                           -
  360. c                                                                     -
  361.     1081 ---------+---------+---------+---------+---------+---------+ 1140
  362. d                                                                     -
  363. e                                                                     -
  364. f          *  T  G  C  R  A  C  G  K  D  K  T  A  L  M                -
  365.  
  366.                              B                   B  AH
  367.                     ANNS     s                   s  ai   F
  368.                     vccm     a                   a  tn   o
  369.                     aiia     W                   H  I4   k
  370.                     IIII     I                   I  II   I
  371.                        /                             /    
  372.          TAGGGAGACTGCCCGGGTGAACCGGAGGAAGGTGGGGATGACGTCAAATCATCATGCCCC
  373.     1141 ---------+---------+---------+---------+---------+---------+ 1200
  374.          ATCCCTCTGACGGGCCCACTTGGCCTCCTTCCACCCCTACTGCAGTTTAGTAGTACGGGG
  375.  
  376. a                                                                     -
  377. b                                             M  T  S  N  H  H  A  P  -
  378. c                                                             M  P  L -
  379.     1141 ---------+---------+---------+---------+---------+---------+ 1200
  380. d                                                     *  I  M  M      -
  381. e                                                                     -
  382. f                                                                     -
  383.  
  384.                          A                                     B
  385.                          f  B                      T           s
  386.                          l  sP                     a           t
  387.                          I  am                     q           E
  388.                          I  Al                     I           I
  389.                          I  II                     I           I
  390.                              /                                  
  391.          TTATGATTTGGGCTACACACGTGCTACAATGGCGTAAACAAAGGGAAGCAAGTGGGTGAC
  392.     1201 ---------+---------+---------+---------+---------+---------+ 1260
  393.          AATACTAAACCCGATGTGTGCACGATGTTACCGCATTTGTTTCCCTTCGTTCACCCACTG
  394.  
  395. a                                                                     -
  396. b         Y  D  L  G  Y  T  R  A  T  M  A  *                          -
  397. c          M  I  W  A  T  H  V  L  Q  W  R  K  Q  R  E  A  S  G  *    -
  398.     1201 ---------+---------+---------+---------+---------+---------+ 1260
  399. d                                                                     -
  400. e                                                                     -
  401. f                      *  V  R  A  V  I  A  Y  V  F  P  L  L  H  T  V -
  402.  
  403.                  H                   BB
  404.                 Bi                   ss
  405.                 pn                   mm
  406.                 l4                   AB
  407.                 II                   II
  408.                  /                    /
  409.          CATGAGCAAATCTCAAAAATAACGTCTCAGTTCGGATTGTAGTCTGCAACTCGACTACAT
  410.     1261 ---------+---------+---------+---------+---------+---------+ 1320
  411.          GTACTCGTTTAGAGTTTTTATTGCAGAGTCAAGCCTAACATCAGACGTTGAGCTGATGTA
  412.  
  413. a                                                                     -
  414. b         M  S  K  S  Q  K  *                                      M  -
  415. c                                                                     -
  416.     1261 ---------+---------+---------+---------+---------+---------+ 1320
  417. d                                                                     -
  418. e                                    *  N  P  N  Y  D  A  V  R  S  C  -
  419. f          M                                                     *  M -
  420.  
  421.                 H                H
  422.                 iT             N iT                    X    ANNS
  423.                 nf             r nf                    m    vccm
  424.                 fi             u fi                    n    aiia
  425.                 II             I II                    I    IIII
  426.                  /                /                            /
  427.          GAAGCTGGAATCGCTAGTAATCGCGAATCAGAATGTCGCGGTGAATACGTTCCCGGGTCT
  428.     1321 ---------+---------+---------+---------+---------+---------+ 1380
  429.          CTTCGACCTTAGCGATCATTAGCGCTTAGTCTTACAGCGCCACTTATGCAAGGGCCCAGA
  430.  
  431. a                                                                     -
  432. b         K  L  E  S  L  V  I  A  N  Q  N  V  A  V  N  T  F  P  G  L  -
  433. c                                        M  S  R  *                   -
  434.     1321 ---------+---------+---------+---------+---------+---------+ 1380
  435. d                       *  Y  D  R  I  L  I  D  R  H  I  R  E  R  T   -
  436. e         S  A  P  I  A  L  L  R  S  D  S  H  R  P  S  Y  T  G  P  D  -
  437. f                                                                     -
  438.  
  439.          B  U
  440.          s Rb                DNS                B
  441.          r sa                sct                s
  442.          G aC                aoy                m
  443.          I II                III                I
  444.             /                 //                 
  445.          TGTACACACCGCCCGTCACACCATGGGAGTTGGGAATGCCCGAAGTCTGTGACCCAACCG
  446.     1381 ---------+---------+---------+---------+---------+---------+ 1440
  447.          ACATGTGTGGCGGGCAGTGTGGTACCCTCAACCCTTACGGGCTTCAGACACTGGGTTGGC
  448.  
  449. a                                                                     -
  450. b         V  H  T  A  R  H  T  M  G  V  G  N  A  R  S  L  *           -
  451. c                                           M  P  E  V  C  D  P  T  V -
  452.     1381 ---------+---------+---------+---------+---------+---------+ 1440
  453. d        K  Y  V  G  G  T  V  G  H  S  N  P  I  G  S  T  Q  S  G  V   -
  454. e         Q  V  C  R  G  D  C  W  P  L  Q  S  H  G  F  D  T  V  W  G  -
  455. f                         *  V  M  P  T  P  F  A  R  L  R  H  G  L  R -
  456.  
  457.                                            B  B
  458.                                            f  s
  459.                                            i  r
  460.                                            I  I
  461.          TAAGGAGGGAGCAGCCGAAGG....CTCGATAACTGGGGTGAAGTCGTAACAAGGTATCC
  462.     1441 ---------+---------+---------+---------+---------+---------+ 1500
  463.          ATTCCTCCCTCGTCGGCTTCC....GAGCTATTGACCCCACTTCAGCATTGTTCCATAGG
  464.  
  465. a                                                                     -
  466. b                                                                     -
  467. c          R  R  E  Q  P  K  ?  ?  S  I  T  G  V  K  S  *             -
  468.     1441 ---------+---------+---------+---------+---------+---------+ 1500
  469. d        T  L  L  S  C  G  F  ?  .  E  I  V  P  T  F  D  Y  C  P  I   -
  470. e         Y  P  P  L  L  R  L  ?  ?  R  Y  S  P  H  L  R  L  L  T  D  -
  471. f          L  S  P  A  A  S  P  .  ?  S  L  Q  P  S  T  T  V  L  Y  G -
  472.  
  473.          T
  474.     1501 - 1501
  475.          A
  476.  
  477. a          -
  478. b          -
  479. c          -
  480.     1501 - 1501
  481. d        R -
  482. e          -
  483. f          -
  484.  
  485.  Enzymes that do cut:
  486.  
  487.     AatII   AceIII     AciI   AflIII     AluI     AlwI     ApoI     AvaI
  488.     AvaII     BaeI     BanI     BbvI     BccI    BcefI     BcgI     BfaI
  489.      BfiI     BmgI     BplI   Bpu10I    BsaAI    BsaHI    BsaJI    BsaWI
  490.      BsbI    BscGI     BslI     BsmI    BsmAI    BsmBI    BsmFI    BsoFI
  491.    Bsp24I Bsp1286I    BspEI    BspGI     BsrI    BsrFI    BsrGI    BssSI
  492.    BstEII    Cac8I     CjeI     CjeI    CjePI    CjePI    CviJI    CviRI
  493.      DdeI     DpnI     DsaI     EaeI     EciI    EcoNI EcoO109I    EcoRI
  494.    EcoRII     FauI     FokI    GdiII   HaeIII     HgaI   HgiEII     HhaI
  495.     Hin4I    HinfI     HphI     KpnI    MaeII   MaeIII    MboII     MluI
  496.      MmeI     MnlI     MseI     MspI   MspA1I     MwoI     NciI     NcoI
  497.    NlaIII    NlaIV     NruI     NspI     NspV     PleI     PmlI    PshAI
  498.    Psp5II     RsaI    SacII    SanDI   Sau96I   Sau3AI    ScrFI    SfaNI
  499.      SfcI     SmaI    SnaBI     SspI     StyI     TaqI    TaqII     TauI
  500.      TfiI     ThaI     TseI   Tsp45I  Tsp509I    TspRI Tth111II    UbaCI
  501.      XmnI
  502.  
  503.  Enzymes that do not cut: 
  504.  
  505.      AccI    AflII     AhdI    AlwNI     ApaI    ApaBI    ApaLI     AscI
  506.     AvrII    BamHI    BanII     BbsI   Bce83I     BclI     BglI    BglII
  507.      BpmI Bpu1102I     BsaI    BsaBI    BsaXI    BseRI     BsgI    BsiEI
  508.   BsiHKAI BspLU11I    BspMI    BsrBI    BsrDI   BssHII Bst1107I    BstXI
  509.     BstYI   Bsu36I     ClaI     DraI   DraIII     DrdI    DrdII     EagI
  510.      EarI Eco47III   Eco57I    EcoRV     FseI     FspI     HaeI    HaeII
  511.    HincII  HindIII     HpaI     MscI     MslI     MunI     NarI     NdeI
  512.    NgoAIV     NheI     NotI     NsiI     PacI Pfl1108I    PflMI    PinAI
  513.      PmeI Psp1406I     PstI     PvuI    PvuII     RcaI    RleAI    RsrII
  514.      SacI     SalI     SapI     ScaI    SexAI     SfiI     SgfI    SgrAI
  515.      SpeI     SphI     SrfI Sse8387I Sse8647I     StuI     SunI     SwaI
  516.   Tth111I     VspI     XbaI     XcmI     XhoI
  517.  
  518.  Enzymes excluded; MinCuts: 1  MaxCuts: 4
  519.  
  520.     AciI     AluI     BbvI     BfaI    BsaJI    BscGI     BslI    BsoFI 
  521.    Cac8I     CjeI    CjePI    CviJI    CviRI     DdeI     HphI    MaeII 
  522.   MaeIII     MnlI     MspI     MwoI   NlaIII    NlaIV    ScrFI     TaqI 
  523.     ThaI     TseI   Tsp45I 
  524.